LDH BR

SFBC
UV enzymatic method
KINETIC

PRINCIPLE

Lactate dehydrogenase (LD/LDH) catalyzes the reduction of pyruvate to lactate (P-L) in the presence of reduced nicotinamide adenine dinucleotide (NADH) at pH 7.5. The reaction is monitored kinetically at 340 nm by the rate of decrease in absorbance resulting from the oxidation of NADH to NAD+ proportional to the activity of LD present in the sample.