LDL-CHOLESTEROL DIRECT

DIRECT
Enzymatic colorimetric method
ENDPOINT

PRINCIPLE

The assay is based on a modified polyvinyl sulfonic acid (PVS) and polyethyleneglycolmethylether (PEGME) coupled classic precipitation method with the improvements in using optimized  quantities of PVS/PEGME and selected detergents. LDL, VLDL, and chylomicron (CM) react with PVS and PEGME and the reaction results in inaccessibility of LDL, VLDL and CM by cholesterol oxidase (CHOD) and cholesterol esterase (CHER), whereas HDL reacts with the enzymes.

Addition of R2 containing a specific detergent releases LDL from the PVS/PEGME complex. The released LDL reacts with the enzymes to produce H2O2 which is quantified by the Trinder reaction.

The intensity of the color formed is proportional to the LDLc concentration in the sample.