PROTEIN (URINE AND CSF)

URINE AND CSF
Colorimetric method
ENDPOINT

PRINCIPLE

The method measures the shift in the absortion spectrum from 460 to 600 nm of the complex that occurs at acid pH between pyrogallol red-molibdate (PRM) and the basic amino groups of urine  and cerebrospinal fluid (CSF) proteins. The intensity of the colored complex formed is proportional to the concentration of protein in the sample.