IFCC
UV enzymatic method
KINETIC
PRINCIPLE
Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to oxoglutarate with the formation of glutamate and oxalacetate. The latter is reduced to malate by
malate dehydrogenase (MDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH).
The reaction is monitored kinetically at 340 nm by the rate of decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to the activity of AST present in the sample.
Available files
Cromatest - Product insert
1109000 AST/GOT BR opt. 2x50 ml
1109010 AST/GOT BR opt. 3x100 ml
KR10072 AST/GOT BR opt. 5x40 ml
KR10075 AST/GOT BR opt. 11x40 ml
CT10072 AST/GOT BR opt. 3x50 ml
CT10075 AST/GOT BR opt. 12x50 ml