GOT/GPT color

REITMAN-FRANKEL
Colorimetric method
ENDPOINT

PRINCIPLE

Aspartate aminotransferase (GOT) catalyzes the transfer of the amino group from aspartate to oxoglutarate with the formation of glutamate and oxalacetate.

L-Aspartate + 2-Oxoglutarate L-Glutamate + Oxalacetate

Alanine aminotransferase (GPT) catalyzes the transfer of the amino group from alanine to oxoglutarate with the formation of glutamate and pyruvate.

L-Alanine + 2-Oxoglutarate Glutamate + Pyruvate

The transaminase activity is proportional to the amount of oxalacetate or pyruvate formed over a definite period of time and is measured by the reaction with 2,4-dinitrophenylhydrazine (DNPH) and measurement of the color formed in an alkaline solution.