IFCC
UV enzymatic method
KINETIC
PRINCIPLE
Alanine aminotransferase (ALT/GPT) catalyzes the transfer of the amino group from alanine to oxoglutarate with the formation of glutamate and pyruvate. The latter is reduced to lactate by lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH).
The reaction is monitored kinetically at 340 nm by the rate of decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to the activity of ALT present in the sample.
Available files
1105000 ALT/GPT BR opt. 2x50 ml
1105010 ALT/GPT BR opt. 3x100 ml
KR10052 ALT/GPT BR opt. 5x40 ml
KR10055 ALT/GPT BR opt. 11x40 ml
CT10052 ALT/GPT BR opt. 3x50 ml
CT10055 ALT/GPT BR opt. 12x50 ml