Colorimetric enzymatic method



In this direct method α-amylase catalyzes the hydrolysis of 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNP-G3) substrate at pH 6.0 forming 2-chloro-p-nitrophenol (CNP) and free glycosides.


The reaction is monitored kinetically at 405 nm by the rate of formation of the colored CNP produced, proportional to the activity of the α-amylase in the sample.

Available files

1107005 Amylase MR 5x20 ml

KR10060 Amylase MR 2x30 ml
KR10062 Amylase MR 8x30 ml
CT10060 Amylase MR 2x40 ml
CT10062 Amylase MR 6x40 ml