BILIRUBIN (TOTAL AND DIRECT)

TOTAL and DIRECT
Colorimetric method
END POINT

 

PRINCIPLE

Bilirubin is converted to coloured azobilirubin by diazotized sulfanilic acid and is measured photometrically. Of the two bilirubin fractions in serum –bilirubin-glucuronide and free bilirubin which is bound to albumin– only the former reacts directly, while free albumin reacts after being displaced from protein by an accelerator. The difference of two measurements total bilirubin (with accelerator) and direct bilirubin (without accelerator) enables the calculation of indirect bilirubin.

 

The terms «direct» and «indirect» bilirubin refers exclusively to the reaction characteristics in the presence or absence of an accelerator or solubilizer and are only approximate equivalents of the two bilirubin fractions.

 

Available files

1110005 Bilirubin Direct 2x50 ml
1111010 Bilirubin Total 2x100 ml
1112005 Bilirubin Total and Direct 2x100 ml

KR10082 Bilirubin Direct 5x40 ml
KR10092 Bilirubin Total 5x40 ml
KR10095 Bilirubin Total 11x40 ml
CT10082 Bilirubin Direct 3x50 ml
CT10085 Bilirubin Direct 12x50 ml
CT10092 Bilirubin Total 3x50 ml
CT10095 Bilirubin Total 12x50 ml