TOTAL AND INHIBITED
Enzymatic colorimetric method
KINETIC
PRINCIPLE
Cholinesterase (CHE) catalyzes the hydrolysis of butyrylthiocholine substrate forming butyrate and thiocholine. The latter reduces 5,5´- mercaptobis-2-nitrobenzoic acid (DMNB) to 5-mercapto-2- nitrobenzoate (5-MNBA), a colored compound.
The reaction is monitored kinetically at 405 nm by the rate of formation of the yellow color produced, proportional to the activity of CHE in the sample.
Available files
1119005 Cholinesterase (Total and Inhibited) 30 Test