HbA1c-Turbidimetric

Latex Turbidimetry

PRINCIPLE

This method directly determinates the hemoglobin A1c (HbA1c) in whole blood, using an antigen and antibody reaction. Total hemoglobin and HbA1c compite for the unspecific absortion rate to the latex particles (R1). Mouse ant-human HbA1c monoclonal antibody (R2a) binds to the coated particles with HbA1c. The presence of goat anti-mouse IgG polyclonal antibody (R2b) causes the  agglutination of the particles (complexes).The amount of agglutination is proportional to the concentration of the HbA1c in the sample and can be measured by turbidimetry.