HEMOGLOBIN

TOTAL
Colorimetric method
ENDPOINT

 

PRINCIPLE

 

The Fe (II) of all forms of hemoglobin, with the exception of sulfohemoglobin, is oxidized to the Fe(III) of methemoglobin which, in turn, reacts with ionized cyanide (CN-) to form cyanmethemoglobin, a stable derivate which absorbs at 540 nm. The intensity of the color produced is proportional to the concentration of total hemoglobin in the sample.