LDL-CHOLESTEROL

DIFFERENTIAL PRECIPITATION
Enzymatic colorimetric test
ENDPOINT

 

PRINCIPLE

 

This technique uses a separation method based on the specific precipitation of low-density lipoproteins (LDL) by polyvinyl sulfate in whole serum, sedimentation of the precipitant by  centrifugation, and subsequent test as residual cholesterol of the rest of lipoproteins (VLDL+ HDL) remaining in the clear supernatant.

 

LDL-cholesterol is calculated by substracting the supernatant cholesterol fraccions from the total cholesterol of the sample.