LIPASE

Enzymatic colorimetric method
KINETIC

 

PRINCIPLE

 

The method for the determination of lipase is based on the cleavage of specific chromogenic lipase substrate 1,2-Odilaurylrac- glycero-3-glutaric acid-(6’methyl-resorufin)-ester emulsified in  stabilized micro-particles. In the presence of specific activators of pancreatic lipase as colipase, calcium ions and bile acids, the substrate is con-verted to 1,2-O-dilauryl-rac-glycerol and glutaric acid-6’-methylresorufinester which decomposes spontaneously to glutaric acid and methylresorufin.

 

The increase of absorbance at 580 nm, due to methylresorufin formation, is proportional to the activity of lipase in the sample.