Determination of anti-Brucella antibodies
The Rose Bengal Test or buffered Brucella antigen test (BBA) is a rapid slide agglutination procedure developed for the direct detection of Brucella antibodies in human and animal sera. The bacterial suspension is reactive with both immunoglobulin G and immunoglobulin M antibodies being the former detected earlier (sub-clinical infections) and over a large period during the disease (chronic stage) than the conventional tube agglutination test.
The assay is performed by testing the buffered suspension (pH 3.6) of B. abortus strain coloured with Rose Bengal against unknown sera. The presence or absence of a visible agglutination, indicates the presence or absence of antibodies in the samples tested.