Enzymatic colorimetric method
ENDPOINT
PRINCIPLE
The method is based on the enzymatic hydrolysis of serum or plasma triglyceride to glycerol and free fatty acids (FFA) by lipoprotein lipase (LPL). The glycerol is phosphorylated by adenosin triphosphate (ATP) in the presence of glycerolkinase (GK) to form glycerol-3-phosphate (G-3-P) and adenosine diphosphate (ADP).
G-3-P is oxidized by glycerophosphate oxidase (GPO) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide.
A red chromogen is produced by the peroxidase (POD) catalyzed coupling of 4-aminoantipyrine (4-AA) and phenol with hydrogen peroxide (H2O2), proportional to the concentration of triglyceride in the sample.
Available files
Cromatest - Product insert
1155005 Triglycerides MR 2x50 ml
1155010 Triglycerides MR 4x100 ml
KR10360 Triglycerides MR 2x30 ml
KR10362 Triglycerides MR 8x30 ml
KR10365 Triglycerides MR 18x30 ml
CT10360 Triglycerides MR 2x40 ml
CT10362 Triglycerides MR 6x40 ml
CT10365 Triglycerides MR 10x60 ml