UREA BERTHELOT

Urease/Salycilate
Enzymatic colorimetric method
ENDPOINT

PRINCIPLE

Urea is hydrolyzed by urease into ammonia and carbon dioxide. The ammonia generated reacts with alkaline hypochlorite and sodium salicylate in presence of sodium nitroprusside as coupling agent to yield a green cromophore. The intensity of the color formed is proportional to the concentration of urea in the sample.